: Using the application for the qPCR thermocycler, establish at which cycle the fluorescence intensity crosses a person‐specified threshold. Employing good and detrimental controls as benchmarks, decide on a threshold that Plainly distinguishes amplification from non‐amplification. If a solution of proteinase K (PK) is used for sample collection (Primary Protocol https://www.facebook.com/NewTechFind/posts/pfbid0FDwXLmxi2raDNPcEpRa9jeGJbqTJ9rKxkuvCV6x8ZRC8R3wYhPw7yVXLXMNTF8JFl